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Recorded June 16, 2020, 9:00-10:15 am CDT
No Registration Required

Session Abstract:

Biosensors are analytical medical devices used for the detection of a chemical substance, that combines a biological component with a physicochemical detector. Biosensors usually convert a biological response into an electrical signal. This session will have three invited talks on different biosensors for medical applications.

"Microfluidic Organoid Cultures for Personalizing Cancer Treatment"
Alexander Revzin, Professor, Department of Biomedical Engineering and Physiology, Mayo Clinic

"Microfluidic-Robotic Interface"
Tingrui Pan, Department of Biomedical Engineering, University of California, Davis

"A laser-engraved low-cost microwell array platform for point-of-care colorimetric diagnosis of COVID-19"
Yang Wang, Chinese Academy of Sciences

Session Organizer Bios:

Tianhong Cui, Department of Mechanical Engineering, University of Minnesota
Tianhong Cui is currently a Distinguished McKnight University Professor at the University of Minnesota. He is a Professor in Mechanical Engineering and an Affiliate Senior Member of the graduatefaculty in Department of Electrical Engineering and Department of Biomedical Engineering. He is an Adjunct Professor at Mayo Clinic, a Distinguished Visiting Fellow at the University of Cambridge, and a Distinguished Visiting Professor at University of Paris East. He is a Fellow of American Society of Mechanical Engineering (ASME). His current research interests include MEMS and nanotechnology. He has more than 320 archived publications in scientific journals and prestigious conferences. He is the founding Executive Editor-in-Chief for a Nature journal, Microsystems & Nanoengineering. He is also serving as the founding Editor-in-Chief for the first AAAS/Science Partner Journal titled Research.

Speaker Bios:

Alexander RevzinAlexander Revzin, Professor, Department of Biomedical Engineering and Physiology, Mayo Clinic
Alexander Revzin received B.S. degree in chemical engineering from Wayne State University and Ph.D. in chemical engineering from Texas A&M University where he worked on enzyme-based electrochemical biosensors. Subsequently, he spent two years as a postdoctoral fellow in Harvard Medical School/Massachusetts General Hospital with Mehmet Toner, developing micropatterned surfaces for cell analysis. Revzin joined Department of Biomedical Engineering at University of California, Davis in 2004 and rose to the rank of Professor. He moved to Mayo Clinic in Rochester Minnesota in October 2016.

The Revzin laboratory pursues research projects at the intersection of cell biology, biosensors, microfabrication and surface science. From November 2012 until July 2014 Revzin served as a Program Director at the National Science Foundation (USA) overseeing research funding in the area of nano-biosensing. Revzin was a Chancellor Fellow at UC Davis in 2012 and was inducted as a fellow of AIMBE in 2015.

Tingrui PanTingrui Pan, Department of Biomedical Engineering, University of California, Davis
Prof. Tingrui Pan is a Professor in the Department of Biomedical Engineering at UC Davis. Leading the Micro-Nano Innovations (MiNI) Group, his research interests span a wide range of topics in bioengineering, including tactile sensing and flexible electronics, microfluidics and lab-on-a-chip systems, laboratory automation, robotic medicine. He is an elected Fellow of American Institute for Medical and Biological Engineering (AIMBE) and Royal Society of Chemistry (RSC).


Yang WangYang Wang, Institute of Electronics, Chinese Academy of Sciences
Yang Wang, Ph.D, is currently a Research Associate at the Institute of Electronics, Chinese Academy of Sciences. He received his doctoral degree in microelectronics in 2019 and his MS degree in bioelectronics in 2016 at Chinese Academy of Sciences. His previous experience includes working in the Biomedical Engineering group at University of Glasgow, UK. His research interests include nucleic acid testing, electrochemical biosensors and paper-based microfluidics.


Presentation Abstracts:

"Microfluidic Organoid Cultures for Personalizing Cancer Treatment"
Our lab has a long-standing interest in developing microfluidic and biosensing technologies for analysis of cells and biological fluids. This presentation will focus on the use of microfluidic devices for cultivation of cancer organoids. It will highlight the advantages of microfluidic cancer cultures and will describe how microfluidic automation may enable testing of anti-cancer drugs based on small cell input. In addition, this presentation will describe an automated microfluidic droplet generation device for analysis of cell function in sub-nanoliter volumes of media. Finally, a microfluidic platform integrating both cell culture and droplet-based cell analysis modules will be described. A microfluidic platform for creating cancer organoids based on patient biopsies and for testing patient-specific drug responses will improve precision of cancer treatment by allowing oncologists to tailor anti-cancer therapy to an individual patient.

"Microfluidic-Robotic Interface"
Microfluidic devices and sensors have been increasingly used for many emerging biological and clinical applications. However, laboratory automation of such delicate devices has lagged behind due to the lack of world-to-chip (macro-to-micro) interfaces. In this talk, we have presented the first pipette-free robotic-microfluidic interface using a microfluidic-embedded container cap, referred to as a Microfluidic Cap-to-Dispense (µCD), to achieve a seamless integration of liquid handling and robotic automation without any traditional pipetting steps. The μCD liquid handling platform utilizes the high accuracy and high flexibility of the robotic system to recognize, capture and position; and then using microfluidic adaptive printing to achieve high-precision on-demand liquid operations.

"A laser-engraved low-cost microwell array platform for point-of-care colorimetric diagnosis of COVID-19"
The point-of-care and sensitive detection of SARS-CoV-2 is of great importance for inhibiting the current pandemic of COVID-19. Here we report simple yet efficient to implement a novel microwell array platform for point-of-care colorimetric diagnosis of SARS-CoV-2. To improve the detection specificity, we synthesized a group of six primers to identify the conserved regions of the ORF1ab gene of SARS-CoV-2. A low-cost polymeric chip with laser-engraved microwell array was developed for the reaction between the primers and the respiratory swab RNA extracts based on reverse transcriptase loop-mediated isothermal amplification (RT-LAMP). The microwell was designed into conical-shape, which resulted in expedited amplification speed and colorimetric read-out with naked eyes within 25 minutes, significantly quicker than the tubing platform (45 minutes). We validated the device with 113 clinical samples, from 87 PCR-positive and 26 PCR-negative patients. The experimental results demonstrated both high sensitivity (95.4 %) and high specificity (92.3 %) as compared with RT-qPCR assay. This device is a valued addition to point-of-care testing (POCT) toolbox for rapid and user-friendly diagnosis of COVID-19.



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